RT-PCR Test Process
As we know, the Covid19 pandemic is rapidly growing worldwide, and there are an ascending number of cases of COVID-19 globally. Because of this there’s a rise in discussion of the certain medical terminologies that are used for the detection of COVID-19 like RT-PCR test, TruNat test, Serology blood test, antibody test and many more. By the statement of regulatory authorities like FDA, Food & Agriculture Organization of the United Nations (FAO), RT-PCR (Reverse Transcription Polymerase Chain Reaction) is considered as one of the most specific laboratory testing method to detect current Covid19 Virus.
In Addition, there are also other tests also used to detect the existence of the virus in peoples. Thus, with a growing count of individuals getting infected with this disease there’s a rise within the number of persons getting tested for RT-PCR Test. In many states, New Delhi as well it’s free of cost and needs no prescription for the testing.
RT-PCR Test for Covid 19?
PCR represent Polymerase chain reaction was introduced to spot a little fragment of virus and make multiple copies, making detection easier. PCR is ideal in examining COVID-19 virus and other viral diseases. Learn how this test works.
RT-PCR Test represent Reverse Transcription Polymerase Chain Reaction. It is a molecular form symptomatic testing method. An RT-PCR is a laboratory test that unite Reverse Transcription of RNA into DNA for the detection of covid-19 virus. (RT)-PCR test is recommended by WHO, using for the detection of COVID-19 virus. This test can quickly tell if someone accommodate the virus (5 to 7 hours). Researchers use an enzyme that transform the genetic material RNA within the virus to DNBA through a process known as Reverse Transcription. An RT-PCR test procedure uses throat or nose swabs to spot COVID-19 viruses. It also detects the virus even if the load is less.
RT-PCR is the leading and sensitive method for mRNA detection. It discloses the presence of a specific genetic material of the virus or any pathogen. RT-PCR is filled with fluorescent dyes as markers. RT-PCR helps researcher to examine the results at once.
The vital benefit of this RT-PCR testing procedure is its ability to give instant results.
Why Take Covid 19 RT-PCR Test?
Well, there is no particular vaccine or specific treatment for COVID-19. Some Medical care sure can help to relieve the symptoms. Antibiotics typically do not work in reverse to viruses; they only work out on bacterial infections. COVID-19 is generated by a virus, so antibiotics don’t work. Hence, antibiotics will not be used as prevention or treatment of COVID-19. They must only be used under supervision by a physician to treat a bacterial infection.
If you’re not in a locality where COVID-19 is transmitting, or if you’ve not travelling from one of those areas or haven’t been in close contact with someone who has and is feeling not well, your possibility of getting it is low for now. However, it’s acceptable that you might be feel disturbed and anxious about things going around. It’s a genuine idea to urge the facts to help you determine your risks so as that you’re going to take rational precautions.
In the middle of a surge in COVID-19 cases, the Maharashtra government earlier in the week had demanded the Centre to reduce the cycle threshold (CT) value cut-off from 35 to 24 for positivity rate of the RT-PCR test. They had asked to reduce the CT value for a lesser count of patients who come under the positive group.
But some Days later, the Indian Council of Medical Research (ICMR) denied the Maharashtra government’s request. While replying to them, the ICMR said, “performing a CT value cut-off of 24 isn’t advisable, as this may cause missing of many infectious patients and enhance disease transmission. CT value threshold helps to spot whether a patient is COVID positive or not.”
How to Calculate CT Value?
The calculation of CT Value suggests igenetic Diagnostics’ that offer Cycle Threshold (CT) value on Reverse Transcription-Polymerase Chain Reaction (RT-PCR) test reports. When the sample is taken from the patient, RNA is extracted and treated with a polymerase enzyme. A complementary DNA is pull off from an initial RNA. Next, the DNA can effortless be raise by engaging a polymerize chain reaction to make billions of copies of a fraction.
The DNA calculate in real time by fluorescein signals, through which it helps to measure the CT Value. Meantime, within the exponential stage, fluorescent signals data is pull off which is that the initial amount of the target DNA. Keep in mind: LOWER the CT VALUE equals to HIGHER viral load and HIGHER CT VALUE equals to LOWER viral load.
Pulse Oximeter detect COVID-19 at Home
The Pulse Oximeter is basically used in emergency rooms and hospitals in critical care. The pulse oximeter is a small device looks like a clip bind to a part such as toes or an earlobe. It most likely placed on a finger.
Pulse oximeter is basically a painless and bloodless test and that helps to calculate person oxygen saturation level, or the oxygen levels in your blood. It can instantly spot even little changes. It shows how smoothly oxygen is being carried to the limit furthermost from your heart, counting the legs and the arms as well.
The aim of pulse oximeter is to calculate how good your heart is pumping oxygen through your body.
Summary
At last, RT-PCR test is the centrepiece in determining COVID-19. The Reason why this test is supreme is because to its responsiveness, relevancy, and achievability. Schedule of PCR test in reference to symptoms, will restrict of detection. Sample gather site location is to be consider. New invention in RT-PCR to reverse are mainly centre on all sides, simple procedure, faster turnaround times, and reduce in the use of materials.
This is all about the RT-PCR Test and Cycle Threshold Value for determining if you are COVID-19 positive or not. For more information on covid-19, stay updated on our blog.